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4 edition of Characterization of a neural-specific inducible genetic system in transgenic mice found in the catalog.

Characterization of a neural-specific inducible genetic system in transgenic mice

Jeffrey R. Gingrich

Characterization of a neural-specific inducible genetic system in transgenic mice

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  • 11 Currently reading

Published by National Library of Canada = Bibliothèque nationale du Canada in Ottawa .
Written in English


Edition Notes

SeriesCanadian theses = Thèses canadiennes
The Physical Object
FormatMicroform
Pagination1 microfiche.
ID Numbers
Open LibraryOL20308932M
ISBN 100612292746
OCLC/WorldCa46584266

Transgenic mice — Laboratory Interferon-inducible ES cell expression systems --Transgenic studies in the mouse: improving the technology towards a conditional temporal and spatial approach -- In vivo libraries of large insert transgenic mice for genetic mapping -- Epigenetic effects on transgene expression -- Positional-candidate cloning. Genetically modified mouse. A genetically modified mouse in which a gene affecting hair growth has been knocked out (left), shown next to a normal lab mouse.


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Characterization of a neural-specific inducible genetic system in transgenic mice by Jeffrey R. Gingrich Download PDF EPUB FB2

Gene Expr Patterns. Jan; doi: / Epub Oct Characterization of a new, inducible transgenic mouse model with GFP expression in melanocytes and their by: 4. More recently, the generation and characterization of transgenic mice carrying Cre recombinase under the control of constitutive liver-specific promoters have been described[].

These investigators used the constructs in which either mouse albumin regulation elements and α-fetoprotein enhancers or promoters and upstream enhancers of rat albumin gene were used to drive Characterization of a neural-specific inducible genetic system in transgenic mice book Cre Cited by: 6.

Unlike existing Notch transgenic strains, these transgenic mice contain the endogenous human NOTCH1 promoter, and thus are only expressed in tissues were Notch1 is normally present. Moreover, expression of the transgene is Cre-inducible, and thus control of this system can be finely by: 4.

Less commonly used systems to control gene expression in transgenic mice take advantage of the ligand-binding domain of the progesterone receptor, and the lac and GAL4 inducible systems.

The tetracycline-regulated transgenic models are typically designed to activate the expression of the gene of interest in a specific cell type at a specific point in by: distantly related host cells. A heat shock inducible system was also developed for use in transgenic mice (Kothary et al., ).

Mice were generated that expressed the E. coli B- galactosidase (P-gal) gene under the control of the promoter from the mouse heat shock gene, hspCited by: 1.

In summary, by combining existing tissue-specific Cre-transgenic mouse strains to define spatial, and the tetracycline-inducible system to define temporal aspect of transgene expression, we have developed a versatile system which allows quick and efficient investigation of multiple gene by:   Fcγ receptor (FcγR) engagement is pivotal for many effector functions of macrophages, polymorphonuclear neutrophils (PMN), and natural killer (NK) cells.

Mice transgenic for the A and B isoforms of human (h) FcγRIII on macrophages, PMN, and NK cells were constructed to permit the study of mechanisms and potential in vivo strategies to utilize the cytotoxic effector and antigen-presenting Cited by: 7.

@description. Projection BDA/AAV Comparison Transgenic Characterization Reference Data Brain Explorer Documentation Help BDA/AAV Comparison Transgenic Characterization Reference Data Brain Explorer Documentation Help.

Later, amyloid precursor protein (APP) and its gene sequence were also identified. Alzheimer’s disease is more prevalent in some families, clearly indicating a genetic basis for this disease. Transgenic mice were developed by introducing amyloid precursor gene. Techniques in Molecular Biology (to study the function of genes) Analysis of nucleic acids: Generation of transgenic mice and knock-out mice aLoss of function `Transgenic mouse aFlavours `Conditional (inducible system) `Tissue-specific.

Mutations: types and causes aAn organism’s genotype is its entire set of genes and may denote. It would thus be highly advantageous if the expression of a particular gene could be restricted both temporally and spatially through the use of an inducible genetic system.

This paper describes the various inducible genetic expression systems developed for use in mammalian cells, with particular emphasis on their application in the nervous system of transgenic by:   We will give an overview of available CreER T2 transgenic mouse lines and present protocols that detail the generation of experimental mice for inducible gene knockout studies, the induction of recombination by tamoxifen treatment, and the analysis of the quality and quantity of recombination by reporter gene and target gene studies.

Most of the protocols can also be used as general guidelines for the generation and characterization of Cre/lox-mediated genome modifications in by: A transgenic animal is one that transgene.

The foreign gene inserted is called. What are the two widely used methods of producing transgenic mice. controlling the exact genetic background and quick reproduction with many offspring.

Usefulness of transgenic mice. kills lymphocytes but allows other cells to live. "An authoritative manual covering all aspects of the production and analysis of mouse mutants and transgenic mice. Initial chapters discuss animal husbandry and cryopreservation methods.

This is followed by discussions of various genetic and physical mapping techniques, as well as applications of fluorescence in situ hybridisation (FISH).5/5(1). To establish a genetic tool for manipulating the neural stem/progenitor cell (NSC) lineage in a temporally controlled manner, we generated a transgenic mouse line carrying an NSC-specific nestin promoter/enhancer expressing a fusion protein encoding Cre recombinase coupled to modified estrogen receptor ligand-binding domain (ER T2).Cited by: Transgenic Mice with Defined Combinations of Drug Inducible Reprogramming Factors Styliani Markoulaki 1,3, Jacob Hanna 1,3, Caroline Beard 1, Bryce W.

Carey 1,2, Albert W. The choice of system employed is dependent on the particular aim of the investigation, and may influence the final result. The inducible and conditional promoter system represents a useful experimental approach for the development of transgenic animals and the precise examination of Cited by: 7.

A genetically modified mouse or genetically engineered mouse model (GEMM) is a mouse (Mus musculus) that has had its genome altered through the use of genetic engineering techniques.

Genetically modified mice are commonly used for research or as animal models of human diseases, and are also used for research on genes. Together with patient-derived xenografts (PDXs), GEMMs are. Inducible Gene Targeting in Mice Using the Cre/lox System Brian Sauer Laboratory of Biochemistry and Metabolism, National Institute of Diabetes, Digestive and Kidney Disease, National Institutes of Health, Build Room 9N, Bethesda, Maryland E-mail: [email protected] genetically alter the mouse genome with nucleotideFile Size: KB.

Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases.

Composed in the highly successful Methods in Molecular Biology series. A growing number of CreER T2 transgenic mice further allows for inducible inactivation of floxed alleles in adult mice upon administration of tamoxifen. This chapter covers the design and construction of loxP flanked alleles and refers to the vectors, ES cells, and mice generated by the European conditional mouse mutagenesis (EUCOMM) by: Protocol used to Create Transgenic Mice using CRISPR-Cas9.

Dongwuxue Yanjiu. Jul 18; 37(4): – Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems. Figure 1. Schematic of using CRISPR/Cas9 genome editing on mouse embryos to create transgenic mice.

An inducible transgenic mouse breast cancer model for the analysis of tumor antigen speci c CD8 + T-cell responses Michael Bruns 1, Jara Wanger 1,4, Olaf Utermöhlen 2, Wolfgang Deppert 1,3.

Inducible Gene Expression with Tetracycline Transactivator System Gene Targeting by Inducible Activation System Tissue Specific Control of Gene Expression 3.

Transgenic Mice and the Immune System RAG-Deficient Blastocyst Complementation Transgenic Mice in the Study of Immunological Diseases X-Linked SCID wild-type mice, i.e., sCJD, and in these cases transgenic mouse panels using mice in which the murine PrP sequence has been replaced by that of another species have proved to be useful (Bishop et.

The Cre-loxP system is a conditional gene targeting technique used to generate genetic alterations in mice. Unlike methods that alter gene expression in the germ line, and hence in all cell and tissue types, the Cre-loxP system permits cell and/or tissue specific gene by: 1.

• Transgenic mice have significantly contributed to the understanding of molecular biology, genetics, immunology and cancer, besides creating animal models for several human genetic diseases. 3 4. GENERAL PROCEDURE FOR PRODUCING TRANSGENIC MICE: • There are three methods for introducing a transgene into mice: i.

Applications of Transgenic and Knockout Mice in Alcohol Re s e a rc h Barbara J. Bowers, Ph.D. Multiple genetic and environmental factors contribute to the development of alcoholism. Researchers attempting to elucidate the roles of specific genes in alcoholism risk have benefited from advances in genetic Size: KB.

Behavioral Characterization of Transgenic and Knockout Mice (J. Crawley). Genetic Analysis of Receptor Phenotypes (S. Bultman, et al.). Immortalization of Cell Lines from Transgenic and Knockout Mice by Viral Transformation (J. Chou). The VPDependent Binary System for Inducible Gene Expression in Transgenic Mice (C.

Kappen).Author: Domenico Accili. Mouse Model. Mouse models can be considered as the starting point for investigating a certain basic principle, without having an aim to translate it into humans, just like other non-human models such as yeast, fruit fly or zebrafish, depending on the nature of the question in study: for example, studying the mechanism of heat shock proteins in the antigen presentation process or understanding.

Gene expression may occur in unexpected ectopic sites when diverse genetic elements are juxtaposed as chimeric genes in transgenic mice. To determine the specific contribution of the promoter and reporter gene in ectopic expression, we have analyzed the expression of 14 different fusion genes in transgenic by: Other cell types within the central nervous system (CNS) are also susceptible to transformation by SV40, as illustrated by transgenic mice carrying the SV40 early region genes in association with an enhancer/promotor region from adenovirus E1A (type 5) (Kelly et al., ).Two such mice showed signs of altered equilibrium and hind-leg paralysis at 3–4 months of age, and necropsy revealed Author: Albee Messing.

Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, Zeng H.

Citation: Wu GS, Jiang M, Liu Y-H, Nagaoka Y, Rao NA () Phenotype of Transgenic Mice Overexpressed with Inducible Nitric Oxide Synthase in the Retina.

PLoS ONE 7(8): e doi Humanized mice are a subset of transgenic, you're correct. Often, when people refer specifically to "humanized mice" they are referring to a strain which not only expresses a human sequence, but has the endogenous mouse genes knocked out and the homologous human sequences knocked into the endogenous locus to preserve genomic context and promoter elements.

Khalid Iqbal is the editor of Alzheimer's Disease: Advances in Etiology, Pathogenesis and Therapeutics, published by Wiley.

Sangram S. Sisodia is the editor of Alzheimer's Disease: Advances in Etiology, Pathogenesis and Therapeutics, published by Wiley.

Bengt Winblad is the editor of Alzheimer's Disease: Advances in Etiology, Pathogenesis and Therapeutics, published by Wiley. The „Tet-Off-System“ developed by Dr. Bujard (ZMBH Heidelberg) however allows the generation of inducible transgenic mouse models and flexibility in the selection of the targeted brain regions.

Gene expression may occur in unexpected ectopic sites when diverse genetic elements are juxtaposed as chimeric genes in transgenic mice. To determine the specific contribution of the promoter and reporter gene in ectopic expression, we have analyzed the expression of 14 different fusion genes in transgenic mice.

Chimeric genes containing the mouse metallothionein-I promoter linked to either Cited by: The Double homeobox 4 (DUX4) gene is an important regulator of early human development and its aberrant expression is causal for facioscapulohumeral muscular dystrophy (FSHD).

The DUX4-full length (DUX4-fl) mRNA splice isoform encodes a transcriptional activator; however, DUX4 and its unique DNA binding preferences are specific to old-world by: The Transgenic Mouse System Integration and modification of genes into the mouse genome serve to establish experimental systems that are critical to study normal as well as altered gene expression.

Such studies can serve to identify genes essential for normal development and generate animal models for the study of human diseases. duced in the first part of the “Transgenic Mouse Technology in Skin Biology” Research Techniques Made Simple article (Scharfenberger et al., ).

However, many complete and tissue-specific Cre-mediated knockout mice result in embryonic or early perinatal death, thereby precluding the analysis of gene function in different cell types and in.Synthesis of erythropoietin, the primary humoral regulator or erythropoiesis, in liver and kidney is inducible by anemia or hypoxia.

Analysis of human erythropoietin gene expression in transgenic mice revealed that sequences located kilobases 5{prime} to the gene direct expression to the kidney, whereas sequences within the immediate 3{prime}-flanking region control hepatocyte-specific.

Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by Book Edition: 1.